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confocal fluorescence microscopy a1  (Nikon)

 
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    Nikon confocal fluorescence microscopy a1
    Confocal Fluorescence Microscopy A1, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/confocal fluorescence microscopy a1/product/Nikon
    Average 90 stars, based on 1 article reviews
    confocal fluorescence microscopy a1 - by Bioz Stars, 2026-03
    90/100 stars

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    Preparation and characterization of the nanoplatelet vesicles (NPVs). (A) Schematic diagram of NPV preparation. (B) Diameter of platelets (PLTs) and NPVs. (C) Zeta potential of PLTs and NPVs (n = 3). (D) Cryo-transmission electron <t>microscopy</t> (cryo-TEM) images of NPVs; scale bars = 100 nm. (E) Stability of PLTs and NPVs. (F) Coomassie brilliant blue staining and Western blot analysis of PLTs and NPVs. (G) Quantification of IL-1β and IL-6 from PLTs and NPVs with or without thrombin (n = 4). (H–J) Immunofluorescence staining (H) and quantification of the mean <t>fluorescence</t> intensity of iNOS (I) and ARG1 (J) before NPV treatment; scale bars = 50 μm (n = 5). (K) Fluorescence confocal microscopy image of NPV uptake; scale bars = 5 μm. (L) DiI-positive BMSCs were detected by flow cytometry after treatment with NPVs (labeled with DiI), and the proportion of DiI-positive BMSCs was quantified (n = 3). BMSCs were pretreated separately with different endocytosis-related inhibitors for 0.5 h. (M) DiI-positive BMSCs were detected by flow cytometry after treatment with NPVs or PLTs (labeled with DiI), and the proportion of DiI-positive BMSCs was quantified (n = 3). Differences were considered significant when the p value was <0.05.
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    Preparation and characterization of the nanoplatelet vesicles (NPVs). (A) Schematic diagram of NPV preparation. (B) Diameter of platelets (PLTs) and NPVs. (C) Zeta potential of PLTs and NPVs (n = 3). (D) Cryo-transmission electron <t>microscopy</t> (cryo-TEM) images of NPVs; scale bars = 100 nm. (E) Stability of PLTs and NPVs. (F) Coomassie brilliant blue staining and Western blot analysis of PLTs and NPVs. (G) Quantification of IL-1β and IL-6 from PLTs and NPVs with or without thrombin (n = 4). (H–J) Immunofluorescence staining (H) and quantification of the mean <t>fluorescence</t> intensity of iNOS (I) and ARG1 (J) before NPV treatment; scale bars = 50 μm (n = 5). (K) Fluorescence confocal microscopy image of NPV uptake; scale bars = 5 μm. (L) DiI-positive BMSCs were detected by flow cytometry after treatment with NPVs (labeled with DiI), and the proportion of DiI-positive BMSCs was quantified (n = 3). BMSCs were pretreated separately with different endocytosis-related inhibitors for 0.5 h. (M) DiI-positive BMSCs were detected by flow cytometry after treatment with NPVs or PLTs (labeled with DiI), and the proportion of DiI-positive BMSCs was quantified (n = 3). Differences were considered significant when the p value was <0.05.
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    Image Search Results


    Preparation and characterization of the nanoplatelet vesicles (NPVs). (A) Schematic diagram of NPV preparation. (B) Diameter of platelets (PLTs) and NPVs. (C) Zeta potential of PLTs and NPVs (n = 3). (D) Cryo-transmission electron microscopy (cryo-TEM) images of NPVs; scale bars = 100 nm. (E) Stability of PLTs and NPVs. (F) Coomassie brilliant blue staining and Western blot analysis of PLTs and NPVs. (G) Quantification of IL-1β and IL-6 from PLTs and NPVs with or without thrombin (n = 4). (H–J) Immunofluorescence staining (H) and quantification of the mean fluorescence intensity of iNOS (I) and ARG1 (J) before NPV treatment; scale bars = 50 μm (n = 5). (K) Fluorescence confocal microscopy image of NPV uptake; scale bars = 5 μm. (L) DiI-positive BMSCs were detected by flow cytometry after treatment with NPVs (labeled with DiI), and the proportion of DiI-positive BMSCs was quantified (n = 3). BMSCs were pretreated separately with different endocytosis-related inhibitors for 0.5 h. (M) DiI-positive BMSCs were detected by flow cytometry after treatment with NPVs or PLTs (labeled with DiI), and the proportion of DiI-positive BMSCs was quantified (n = 3). Differences were considered significant when the p value was <0.05.

    Journal: Bioactive Materials

    Article Title: Sequential simulation of regeneration-specific microenvironments using scaffolds loaded with nanoplatelet vesicles enhances bone regeneration

    doi: 10.1016/j.bioactmat.2025.04.018

    Figure Lengend Snippet: Preparation and characterization of the nanoplatelet vesicles (NPVs). (A) Schematic diagram of NPV preparation. (B) Diameter of platelets (PLTs) and NPVs. (C) Zeta potential of PLTs and NPVs (n = 3). (D) Cryo-transmission electron microscopy (cryo-TEM) images of NPVs; scale bars = 100 nm. (E) Stability of PLTs and NPVs. (F) Coomassie brilliant blue staining and Western blot analysis of PLTs and NPVs. (G) Quantification of IL-1β and IL-6 from PLTs and NPVs with or without thrombin (n = 4). (H–J) Immunofluorescence staining (H) and quantification of the mean fluorescence intensity of iNOS (I) and ARG1 (J) before NPV treatment; scale bars = 50 μm (n = 5). (K) Fluorescence confocal microscopy image of NPV uptake; scale bars = 5 μm. (L) DiI-positive BMSCs were detected by flow cytometry after treatment with NPVs (labeled with DiI), and the proportion of DiI-positive BMSCs was quantified (n = 3). BMSCs were pretreated separately with different endocytosis-related inhibitors for 0.5 h. (M) DiI-positive BMSCs were detected by flow cytometry after treatment with NPVs or PLTs (labeled with DiI), and the proportion of DiI-positive BMSCs was quantified (n = 3). Differences were considered significant when the p value was <0.05.

    Article Snippet: Finally, the incorporation rate of EdU was determined via confocal fluorescence microscopy (Nikon, A1, Japan).

    Techniques: Zeta Potential Analyzer, Transmission Assay, Electron Microscopy, Staining, Western Blot, Immunofluorescence, Fluorescence, Confocal Microscopy, Flow Cytometry, Labeling